7VLZ

Crystal structure of the collagenase unit of a Vibrio collagenase from Vibrio harveyi VHJR7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	74.641, 50.573, 90.968
Unit cell angles	90.00, 104.98, 90.00

Refinement procedure

Resolution	33.840 - 1.600
R-factor	0.1671
Rwork	0.166
R-free	0.18720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7esi
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.840	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.046	0.181
Rmeas	0.055	0.213
Rpim	0.029	0.111
Number of reflections	85167	8401
<I/σ(I)>	20.74	5.53
Completeness [%]	98.0
Redundancy	3.4	3.5
CC(1/2)	0.993	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M calcium acetate, 0.1 M imidazole/HCl (pH 8.0), and 10% (w/v) polyethylene glycol 8000