7VLM
crystal structure of anti-CRISPR protein AcrIIA18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-12-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.371, 32.405, 62.594 |
| Unit cell angles | 90.00, 120.37, 90.00 |
Refinement procedure
| Resolution | 31.130 - 1.380 |
| R-factor | 0.1832 |
| Rwork | 0.182 |
| R-free | 0.20230 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19rc6_4061) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Number of reflections | 37360 | 3705 |
| <I/σ(I)> | 16.24 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.991 | 0.905 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 291.15 | PEG 3350, calcium acetate |
