7VLB
Crystal structure of UGT109A1 from Bacillus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-12-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 137.874, 137.874, 135.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.746 - 3.000 |
| Rwork | 0.231 |
| R-free | 0.27540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iir |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.728 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.750 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.078 | 0.618 |
| Rmeas | 0.080 | |
| Number of reflections | 26821 | 1302 |
| <I/σ(I)> | 23 | |
| Completeness [%] | 100.0 | |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | PEG 3350 |
