7VL4
The complex structure of beta-1,2-glucosyltransferase from Ignavibacterium album with methyl beta-D-glucoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.949, 71.650, 130.110 |
| Unit cell angles | 90.00, 105.59, 90.00 |
Refinement procedure
| Resolution | 19.978 - 1.830 |
| Rwork | 0.171 |
| R-free | 0.20850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.569 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.990 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.101 | 0.639 |
| Number of reflections | 126164 | 6096 |
| <I/σ(I)> | 9.4 | 1.8 |
| Completeness [%] | 98.3 | 97.1 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M Tris-HCl (pH 7.5), 0.2M calcium acetate, 20%(w/v) PEG 3350 |
