7VKX
The complex structure of beta-1,2-glucosyltransferase from Ignavibacterium album with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.430, 71.660, 130.150 |
| Unit cell angles | 90.00, 106.00, 90.00 |
Refinement procedure
| Resolution | 48.008 - 1.560 |
| Rwork | 0.165 |
| R-free | 0.19010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.706 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.230 | 1.580 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.072 | 0.559 |
| Number of reflections | 201492 | 10000 |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 95.6 | 98.5 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M Tris-HCl (pH 7.5), 0.2M calcium acetate, 20%(w/v) PEG 3350 |
