7VJL

The crystal structure of FGFR4 kinase domain in complex with N-(5-cyano-4-((2-methoxyethyl)amino)pyridin-2-yl)-7-(2,2,2-trifluoroacetyl)-3,4-dihydro-1,8-naphthyridine-1(2H)-carboxamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-22
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.978690
Spacegroup name	P 21 21 21
Unit cell lengths	53.370, 66.785, 184.174
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.908 - 2.900
R-factor	0.211575321392
Rwork	0.205
R-free	0.27301
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jpj
RMSD bond length	0.003
RMSD bond angle	0.785
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	15232	1491
<I/σ(I)>	10.14
Completeness [%]	99.5
Redundancy	4.4
CC(1/2)	0.915	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.5m NH4H2PO4