7VIV

Crystal structure of the I73R from African Swine Fever Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2020-10-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	H 3
Unit cell lengths	89.360, 89.360, 46.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.820 - 2.000
R-factor	0.1872
Rwork	0.185
R-free	0.22280
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.680	44.680	2.010
High resolution limit [Å]	1.960	8.770	1.960
Rmerge	0.046	0.033	0.875
Rmeas	0.048	0.035	0.925
Rpim	0.015	0.011	0.298
Total number of observations	99802	956	7121
Number of reflections	9855	109	749
<I/σ(I)>	23	47.4	2.5
Completeness [%]	99.9	98.8	99.9
Redundancy	10.1	8.8	9.5
CC(1/2)	1.000	1.000	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	289	0.2 M Li2SO4, 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG3350