7VIU

Crystal structure of Au(200EQ)-apo-R168C/L169C-rHLFr

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-16
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.540562
Spacegroup name	F 4 3 2
Unit cell lengths	180.786, 180.786, 180.786
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.950 - 1.500
Rwork	0.151
R-free	0.16680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dat
RMSD bond length	0.012
RMSD bond angle	1.962
Data reduction software	CrysalisPro (171.40_64.67a)
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.950	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	40835	1955
<I/σ(I)>	24.2
Completeness [%]	99.7
Redundancy	9.8
CC(1/2)	0.999	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Ammonium Sulfate, Cadmium Sulfate