7VIO

Crystal structure of recombinant horse spleen apo-R168H/L169C-Fr

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	F 4 3 2
Unit cell lengths	182.644, 182.644, 182.644
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.310 - 1.500
Rwork	0.175
R-free	0.19340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dat
RMSD bond length	0.015
RMSD bond angle	2.217
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.310	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.028	0.039
Rmeas	0.039	0.055
Rpim	0.028	0.039
Number of reflections	42065	2066
<I/σ(I)>	22.7
Completeness [%]	99.7
Redundancy	1.9	1.9
CC(1/2)	0.997	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Ammonium Sulfate, Cadmium Sulfate