7VGW

Yeast gid10 with Pro-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97942
Spacegroup name	C 2 2 21
Unit cell lengths	86.346, 109.913, 147.461
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.060 - 2.800
R-factor	0.2175
Rwork	0.213
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wzz
RMSD bond length	0.002
RMSD bond angle	0.567
Data reduction software	HKL-2000 (v716.1)
Phasing software	PHENIX (1.19-4085)
Refinement software	PHENIX (1.19-4085)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	17734	17555
<I/σ(I)>	14.93
Completeness [%]	99.5
Redundancy	8.8
CC(1/2)	0.980	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	4.95%(v/v) Isopropanol and 0.33M Ammonium citrate/Ammonium hydroxide pH 8.5