7VGT
Time-resolved serial femtosecond crystallography structure of light-driven chloride ion-pumping rhodopsin, NM-R3: resting state structure with bromide ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL3 |
Synchrotron site | SACLA |
Beamline | BL3 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2019-02-02 |
Detector | MPCCD |
Wavelength(s) | 1.7714 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 104.300, 51.100, 78.400 |
Unit cell angles | 90.00, 131.50, 90.00 |
Refinement procedure
Resolution | 29.359 - 2.100 |
R-factor | 0.1775 |
Rwork | 0.176 |
R-free | 0.20750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b2n |
Data reduction software | CrystFEL (0.63) |
Data scaling software | CrystFEL (0.63) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.800 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 18261 | 929 |
<I/σ(I)> | 9.35 | |
Completeness [%] | 100.0 | |
Redundancy | 213 | |
CC(1/2) | 0.988 | 0.895 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | 38% PEG400, 300 mM K phosphate, and 100 mM HEPES (pH7.0) |