7VG9
Crystal structure of phosphotransbutyrylase from Clostridium acetobutylicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.692, 143.408, 113.279 |
| Unit cell angles | 90.00, 93.99, 90.00 |
Refinement procedure
| Resolution | 30.290 - 2.910 |
| R-factor | 0.182 |
| Rwork | 0.172 |
| R-free | 0.23870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yco |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.692 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 62945 | 2970 |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 95.9 | |
| Redundancy | 3 | |
| CC(1/2) | 0.840 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG3350, lithium sulfate, HEPES |
