7VG5
10,5-methenyltetrahydrofolate cyclohydrolase from Methylobacterium extorquens AM1 with tetrahydrofolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-06-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.322, 49.513, 64.071 |
| Unit cell angles | 90.00, 97.38, 90.00 |
Refinement procedure
| Resolution | 31.790 - 2.250 |
| R-factor | 0.1936 |
| Rwork | 0.190 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o5h |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 6.100 | 2.250 |
| Rmerge | 0.119 | 0.096 | 0.308 |
| Rmeas | 0.142 | 0.116 | 0.382 |
| Rpim | 0.077 | 0.064 | 0.223 |
| Total number of observations | 54337 | ||
| Number of reflections | 18336 | 922 | 877 |
| <I/σ(I)> | 14.3 | ||
| Completeness [%] | 97.7 | 92.8 | 96.1 |
| Redundancy | 3 | 3.2 | 2.5 |
| CC(1/2) | 0.970 | 0.656 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | PEG 3350, magnesium chloride hexahydrate, BIS-TRIS |
