7VG4

10,5-methenyltetrahydrofolate cyclohydrolase from Methylobacterium extorquens AM1 strain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 1
Unit cell lengths	53.743, 67.730, 88.586
Unit cell angles	80.14, 79.31, 79.42

Refinement procedure

Resolution	30.590 - 2.770
R-factor	0.2004
Rwork	0.196
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o5h
RMSD bond length	0.006
RMSD bond angle	1.459
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.770	7.590	2.800
Rmerge	0.069	0.043	0.254
Rmeas	0.098	0.060	0.360
Rpim	0.069	0.043	0.254
Number of reflections	28658	1412	1379
<I/σ(I)>	13.4
Completeness [%]	96.9	95.5	93.2
Redundancy	1.9	1.8	1.8
CC(1/2)		0.986	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	PEG 3350, Tacsimate, Sodium acetate trihydrate