7VFT

Crystal structure of rGGGC(CAG)5GUCC oligo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	110
Detector technology	CCD
Collection date	2018-04-20
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0000
Spacegroup name	H 3 2
Unit cell lengths	47.211, 47.211, 185.984
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.700 - 1.450
R-factor	0.196
Rwork	0.195
R-free	0.20780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yn6
RMSD bond length	0.004
RMSD bond angle	0.929
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (1.13_2988)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.448	1.450
Rmerge	0.042
Rmeas	0.043
Rpim	0.011
Number of reflections	14703	1440
<I/σ(I)>	58.06
Completeness [%]	99.8	100
Redundancy	15.2	13.5
CC(1/2)		0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289.15	100mM HEPES, 1.6M ammonium sulfate, 10% PEG 400