7VFQ
Wild type GltA from Bifidobacterium infantis JCM 1222 complexed with lacto-N-tetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.138, 84.381, 106.688 |
| Unit cell angles | 97.41, 92.25, 89.92 |
Refinement procedure
| Resolution | 47.590 - 1.550 |
| R-factor | 0.1927 |
| Rwork | 0.191 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z8f |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.694 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.590 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.075 | 0.355 |
| Rmeas | 0.106 | 0.502 |
| Rpim | 0.075 | 0.311 |
| Number of reflections | 187561 | 8372 |
| <I/σ(I)> | 5.8 | 2 |
| Completeness [%] | 95.5 | 85.7 |
| Redundancy | 1.8 | 1.7 |
| CC(1/2) | 0.970 | 0.725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 30% (w/v) PEG MME 550, 10mM ZnSO4, 0.1M MES-NaOH (pH 5.5) |
