Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-29 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.660, 206.662, 65.935 |
Unit cell angles | 90.00, 104.83, 90.00 |
Refinement procedure
Resolution | 29.850 - 2.280 |
R-factor | 0.2162 |
Rwork | 0.213 |
R-free | 0.27370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ec1 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.280 | 2.280 |
Number of reflections | 49800 | 2418 |
<I/σ(I)> | 27.5 | |
Completeness [%] | 99.6 | |
Redundancy | 4.6 | |
CC(1/2) | 0.995 | 0.710 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 287 | 0.2 M MgCl2, 0.1 M Tris-HCl (pH 8.5), and 25% (w/v) polyethylene glycol (PEG) 3,350 quick-soaked with 6.14 mM UDP and 2.45 mM 5-mer SD-repeat peptide (DSDSD) |