7VFC

Crystal structure of cPMO2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	39.392, 51.223, 57.133
Unit cell angles	90.00, 99.34, 90.00

Refinement procedure

Resolution	29.820 - 1.210
R-factor	0.1564
Rwork	0.155
R-free	0.17880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h1z
RMSD bond length	0.005
RMSD bond angle	0.808
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.820	1.254
High resolution limit [Å]	1.210	1.210
Number of reflections	67508	67522
<I/σ(I)>	9.9
Completeness [%]	98.8
Redundancy	6.6
CC(1/2)	0.928	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	25% PEG3350,0.1 M Tris-HCl, pH8.5,0.2M ammonium acetate