7VEY
Crystal structure of Cyclosorus parasiticus chalcone synthase 1 (CpCHS1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9798 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.155, 95.462, 246.739 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.123 - 1.900 |
R-factor | 0.1819 |
Rwork | 0.181 |
R-free | 0.20770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dxb |
RMSD bond length | 0.009 |
RMSD bond angle | 0.947 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.123 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 197428 | 12527 |
<I/σ(I)> | 10.96 | |
Completeness [%] | 99.4 | |
Redundancy | 12.4 | |
CC(1/2) | 0.975 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M sodium chloride, 0.1 M Tris-HCl, 20 % w/v PEG 4000 |