7VEW
Crystal structure of bacterial chemotaxis-dependent pectin-binding protein SPH1118 in complex with unsaturated trigalacturonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-18 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.687, 83.657, 148.521 |
| Unit cell angles | 90.00, 101.04, 90.00 |
Refinement procedure
| Resolution | 48.591 - 1.920 |
| Rwork | 0.210 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vev |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.730 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.086 | 0.645 |
| Number of reflections | 108404 | 17122 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.5 | |
| Redundancy | 5.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 22% PEG3000 0.15 M Potassium sodium tartrate 0.1 M HEPES 23.4 mM Unsaturated trigalacturonic acid |
