7VET
Crystal structure of bacterial chemotaxis-dependent pectin-binding protein SPH1118 in a closed conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-18 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.626, 54.998, 112.829 |
| Unit cell angles | 103.03, 97.40, 92.36 |
Refinement procedure
| Resolution | 43.530 - 2.250 |
| Rwork | 0.209 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vev |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.790 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 2.390 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.055 | 0.109 |
| Number of reflections | 53456 | 8527 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 95.9 | |
| Redundancy | 6.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350 0.2 M Ammonium formate 0.0209 mM Polygalacturonic acid sodium |
