7VEQ
Crystal structure of bacterial chemotaxis-dependent pectin-binding protein SPH1118 in an open conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-24 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 50.855, 151.663, 179.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.217 - 1.696 |
| Rwork | 0.168 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vev |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.707 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.217 | 1.740 |
| High resolution limit [Å] | 1.696 | 1.700 |
| Rmerge | 0.086 | 0.555 |
| Number of reflections | 76813 | 10681 |
| <I/σ(I)> | 11.1 | 1.87 |
| Completeness [%] | 97.8 | |
| Redundancy | 5.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 2.0 M Ammonium dihydrogenphosphate 0.1 M Tris-HCl 2.0 mM Unsaturated trigalacturonic acid |
