7VEO
Crystal structure of juvenile hormone acid methyltransferase silkworm JHAMT isoform3 complex with S-Adenosyl-L-homocysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.150, 69.170, 57.820 |
| Unit cell angles | 90.00, 99.98, 90.00 |
Refinement procedure
| Resolution | 56.956 - 2.530 |
| Rwork | 0.192 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7v2s |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.492 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.956 | 2.600 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.083 | 0.856 |
| Rmeas | 0.096 | |
| Rpim | 0.524 | |
| Number of reflections | 18812 | 1399 |
| <I/σ(I)> | 11.9 | 2 |
| Completeness [%] | 96.6 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.15M DL-malic acid pH 7.0, 20% PEG 3350 |
