7VEH

Type I-F Anti-CRISPR protein AcrIF13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	F 2 3
Unit cell lengths	262.771, 262.771, 262.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.530 - 2.950
R-factor	0.238
Rwork	0.236
R-free	0.27030
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.246
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.060
High resolution limit [Å]	2.950	2.950
Number of reflections	31578	3108
<I/σ(I)>	30.66
Completeness [%]	100.0
Redundancy	40.4
CC(1/2)	1.000	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	Sodium malonate