7VED

Crystal structure of GABARAP fused to TEX264 LIR with S272D mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2019-06-04
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1000
Spacegroup name	P 21 21 21
Unit cell lengths	38.364, 82.628, 102.434
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.310 - 2.020
R-factor	0.1895
Rwork	0.188
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.007
RMSD bond angle	0.939
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.310	2.093
High resolution limit [Å]	2.020	2.020
Number of reflections	21898	1990
<I/σ(I)>	14.07	1.68
Completeness [%]	99.3
Redundancy	6.4
CC(1/2)	0.998	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M potassium sodium tartrate, 0.1M sodium citrate pH 5.6, 2.0M ammonium sulfate