7VE5
C-terminal domain of VraR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-12-21 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.99984 |
| Spacegroup name | H 3 |
| Unit cell lengths | 60.349, 60.349, 171.521 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.310 - 2.000 |
| R-factor | 0.2005 |
| Rwork | 0.195 |
| R-free | 0.25490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4if4 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.310 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.060 | 0.402 |
| Number of reflections | 14143 | 1129 |
| <I/σ(I)> | 15.29 | |
| Completeness [%] | 89.2 | |
| Redundancy | 2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 288.15 | 100 mM Sodium cacodylate pH 6.6, 10% PEG 1500 (w/v), 5% PEG 400 (w/v), 200 mM MgCl2 |
