7VE4

C-terminal domain of VraR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-16
Detector	RAYONIX MX300-HS
Wavelength(s)	0.99984
Spacegroup name	P 64
Unit cell lengths	50.963, 50.963, 81.063
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.309 - 1.870
R-factor	0.1645
Rwork	0.162
R-free	0.18650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4if4
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.310	1.930
High resolution limit [Å]	1.870	1.870
Rmerge	0.059	0.510
Number of reflections	9739	842
<I/σ(I)>	29.88
Completeness [%]	98.4
Redundancy	5.7	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris-Hcl pH 8.5, 16% PEG 4000 (w/v), 200 mM MgCl2