7VE3
Structure of the complex of sheep lactoperoxidase with hypoiodite at 2.70 A resolution
Replaces: 3R5QExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.942, 80.179, 76.122 |
| Unit cell angles | 90.00, 102.86, 90.00 |
Refinement procedure
| Resolution | 43.974 - 2.700 |
| Rwork | 0.195 |
| R-free | 0.27690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ikc |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.459 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.974 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 17167 | 1120 |
| <I/σ(I)> | 46 | 10 |
| Completeness [%] | 98.1 | 93 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | Ammonium iodide, PEG 3350, pH6.8 |
