7VCJ

Arginine kinase H227A from Daphnia magna

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979490
Spacegroup name	C 1 2 1
Unit cell lengths	78.152, 58.214, 75.416
Unit cell angles	90.00, 100.48, 90.00

Refinement procedure

Resolution	38.420 - 1.750
R-factor	0.1836
Rwork	0.181
R-free	0.22350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ky2
RMSD bond length	0.008
RMSD bond angle	1.038
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.400	1.770
High resolution limit [Å]	1.750	1.750
Rmerge	0.057	0.290
Number of reflections	33113	31495
<I/σ(I)>	12.5
Completeness [%]	98.4
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.8M Sodium/Potassium phosphate, pH 8.17