7VCH

Crystal structure of Neisseria meningitidis EarP bound TDP-L-Rhamnose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	47.407, 124.402, 127.647
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.550 - 2.850
R-factor	0.2291
Rwork	0.225
R-free	0.30910
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.280
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.550	2.952
High resolution limit [Å]	2.850	2.850
Rmerge	0.113	1.210
Number of reflections	18268	1798
<I/σ(I)>	12.26	1.69
Completeness [%]	99.5
Redundancy	5.4
CC(1/2)	0.997	0.642

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M Sodium cacodylate pH6.5, 1.26M Ammonium sulfate