7VCG
Thaumatin protected by alginate gel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AichiSR BEAMLINE BL2S1 |
Synchrotron site | AichiSR |
Beamline | BL2S1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2021-07-21 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.12 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 57.945, 57.945, 150.235 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.970 - 1.250 |
R-factor | 0.1485 |
Rwork | 0.148 |
R-free | 0.16160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ald |
RMSD bond length | 0.005 |
RMSD bond angle | 0.818 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.910 | 1.290 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.222 | 0.937 |
Rmeas | 0.240 | 0.973 |
Rpim | 0.064 | 0.262 |
Number of reflections | 71712 | 7048 |
<I/σ(I)> | 21.5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.9 | 13.7 |
CC(1/2) | 0.987 | 0.785 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | COUNTER-DIFFUSION | 7.5 | 293 | 1 M sodium tartrate, 0.1 M HEPES |