7VBW
Structure of the GTP-bound AAA+ ATPase domain of the transcriptional regulator GtrR in Burkholderia cenocepacia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 65 |
Unit cell lengths | 107.574, 107.574, 40.436 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.580 - 2.500 |
R-factor | 0.1977 |
Rwork | 0.193 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7vbs |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.580 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 9456 | 917 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 99.9 | |
Redundancy | 13.8 | |
CC(1/2) | 0.997 | 0.546 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 11% (w/v) Polyethylene glycol 6000, 100 mM Tris-HCl pH 8.1 |