7VBR

Fe(II)/(alpha)ketoglutarate-dependent dioxygenase TlxI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-20
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 1
Unit cell lengths	64.403, 64.450, 76.988
Unit cell angles	81.75, 73.70, 74.83

Refinement procedure

Resolution	49.320 - 2.100
R-factor	0.2101
Rwork	0.209
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zm2
RMSD bond length	0.008
RMSD bond angle	0.911
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.320	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.081	0.711
Number of reflections	65297	4485
<I/σ(I)>	9.1	1.5
Completeness [%]	97.7
Redundancy	3.7
CC(1/2)	0.996	0.601

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M Bis-Tris (PH 6.5), 2% (v/v) Tacsimate (PH 6.0), 20% (w/v) PEG 3350