7VBE

1.6 Angstrom Resolution Crystal Structure of SARS-CoV-2 Nucleocapsid dimerization domain, pH 5.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-05-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	103.330, 43.490, 59.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.780 - 1.590
R-factor	0.204343912978
Rwork	0.203
R-free	0.22638
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wji
RMSD bond length	0.006
RMSD bond angle	0.817
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.783	1.630
High resolution limit [Å]	1.590	1.590
Rmerge	0.076
Rpim		1.476
Number of reflections	69247	36711
<I/σ(I)>	8.4
Completeness [%]	99.8
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1 M Sodium citrate tribasic dihydrate pH 5.0, 30% v/v Polyethylene glycol monomethyl ether 550