7VBD

Crystal structure of SARS-Cov-2 nucleocapsid N-terminal domain (NTD) protein,pH8.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-06-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	58.977, 55.133, 84.293
Unit cell angles	90.00, 95.06, 90.00

Refinement procedure

Resolution	28.520 - 1.940
R-factor	0.224596537125
Rwork	0.223
R-free	0.26233
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cdz
RMSD bond length	0.007
RMSD bond angle	0.787
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.540	2.050
High resolution limit [Å]	1.940	1.940
Rmerge	0.330	0.483
Number of reflections	76395	38898
<I/σ(I)>	16.1
Completeness [%]	99.3
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	pH 8.0, 0.05M Tris,38% PEG 4000