7VAA

Crystal structure of MiCGT(W93V/V124F/ F191A/R282H) in complex with UDPs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2021-01-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	91.705, 103.759, 109.713
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.496 - 3.100
R-factor	0.249526368324
Rwork	0.245
R-free	0.28879
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vce
RMSD bond length	0.003
RMSD bond angle	0.769
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.150
High resolution limit [Å]	3.100	3.100
Number of reflections	19961	1362
<I/σ(I)>	8.7
Completeness [%]	100.0
Redundancy	6.6
CC(1/2)	0.896	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% (v/v) PEG3350, 0.1 M Bis-Tris pH 7.0, 200 mM calcium acetate