7V9Z

PaOrn Oligoribonuclease native structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	I 41
Unit cell lengths	82.680, 82.680, 193.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.220 - 1.850
R-factor	0.1649
Rwork	0.164
R-free	0.19180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yta
RMSD bond length	0.006
RMSD bond angle	0.724
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.850	1.850
Rpim	0.047	0.462
Number of reflections	54666	2732
<I/σ(I)>	21.06	1.27
Completeness [%]	99.9	99.5
Redundancy	6.2	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	1.0M (NH4)2HPO4, 0.1M imidazole pH 8.0