7V9R

Crystal Structure of the heptameric EcHsp60

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-06-21
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	140.055, 140.967, 240.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.790 - 3.500
R-factor	0.1855
Rwork	0.184
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mrd
RMSD bond length	0.002
RMSD bond angle	0.453
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.790	3.625
High resolution limit [Å]	3.500	3.500
Rmerge	0.059	0.461
Rmeas	0.067	0.540
Rpim	0.030	0.274
Number of reflections	51897	4277
<I/σ(I)>	14.09	3.11
Completeness [%]	85.5	71.64
Redundancy	4.2	3.2
CC(1/2)	0.998	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.75	293	50 Mm Tris-HCl, 500 mM MgCl2, 10.5% (w/v) PEG1000