7V9Q

Crystal structure of the lanthipeptide zinc-metallopeptidase EryP from saccharopolyspora erythraea in open state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97930
Spacegroup name	P 21 21 21
Unit cell lengths	58.818, 111.577, 156.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.180 - 2.670
R-factor	0.2026
Rwork	0.202
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7v9n
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.720
High resolution limit [Å]	2.660	7.240	2.670
Rmerge	0.194	0.111	0.732
Rmeas	0.205	0.117	0.775
Rpim	0.063	0.037	0.248
Number of reflections	30085	1652	1467
<I/σ(I)>	2.9
Completeness [%]	99.7	99.1	98.9
Redundancy	10	10	9.2
CC(1/2)		0.961	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	291.15	0.1 M Citric acid , pH 3.5, 25% w/v PEG 3350