7V9G

Native BEN4 domain of protein Bend3 with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97884
Spacegroup name	P 62 2 2
Unit cell lengths	200.484, 200.484, 151.274
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.780 - 3.500
R-factor	0.2403
Rwork	0.238
R-free	0.27340
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.520
High resolution limit [Å]	3.400	3.400
Rmerge	0.060	0.060
Number of reflections	23049	2480
<I/σ(I)>	20.9
Completeness [%]	87.0
Redundancy	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	100 mM sodium citrate/Citric acid pH 4.0, 200 mM Sodium citrate tribasic, and 17% PEG 3350.