7V98

Crystal Structure of the Dimeric EcHsp60

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	89.287, 95.378, 128.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.960 - 2.350
R-factor	0.2155
Rwork	0.213
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mrd
RMSD bond length	0.002
RMSD bond angle	0.430
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.061	0.804
Rmeas	0.066	0.904
Rpim	0.026	0.404
Number of reflections	42352	3936
<I/σ(I)>	13.69	2.62
Completeness [%]	91.5	86.3
Redundancy	5.6	4.5
CC(1/2)	0.998	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	400 mM KNaC4H4O6, 21 % w/v PEG 3350