7V97
Arsenic-bound p53 DNA-binding domain mutant V272M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.839, 71.524, 84.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.800 - 2.020 |
| R-factor | 0.235 |
| Rwork | 0.234 |
| R-free | 0.25050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tup |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.207 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 2.070 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.182 | |
| Number of reflections | 53184 | 53184 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 98.5 | |
| Redundancy | 5.1 | |
| CC(1/2) | 0.982 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M Tris pH 8.2, 0.2 M Magnesium chloride, 16% w/v PEG 8000 |
