7V8N

Crystal structure of the PWWP-ARID domain of ARID4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	54.668, 54.818, 180.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.870 - 2.050
R-factor	0.1979
Rwork	0.196
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ljx
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.870	46.870	2.130
High resolution limit [Å]	2.050	4.420	2.050
Rmerge	0.107	0.048	0.907
Rmeas	0.116	0.050	0.963
Number of reflections	34354	3731	2942
<I/σ(I)>	18.5	42.1	2.3
Completeness [%]	98.4	100	86.15
Redundancy	12.46	11.99	9.13
CC(1/2)	0.998	0.999	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	2.4 M ammonium phosphate dibasic, 0.1 M Tris pH8.0