7V7X
Structure of H194A AdaV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 140.311, 140.311, 48.322 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.980 - 2.700 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.23160 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (v1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.980 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 17011 | 2049 |
| <I/σ(I)> | 26.3 | |
| Completeness [%] | 99.8 | |
| Redundancy | 20 | |
| CC(1/2) | 0.999 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.085 M HEPSE sodium pH7.5 1.7% Polyethylene glycol 400 1.7 M Ammonium sulfate 15% v/v Glycerol |
