7V63

Structure of dimeric uPAR at low pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 43 2 2
Unit cell lengths	77.449, 77.449, 275.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.037 - 2.906
R-factor	0.2409
Rwork	0.239
R-free	0.27310
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CRANK
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.900	6.240	2.900
Rmerge	0.126	0.075	0.313
Total number of observations	157976
Number of reflections	17605	2156	906
<I/σ(I)>	7.6
Completeness [%]	90.3	99.1	48.2
Redundancy	9	8.9	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	1.8-2.2 M ammonium sulphate in 50 mM sodium acetate at pH 4.5-5.2