7V5T
Crystal structure of human bleomycin hydrolase C73S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-04-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9732 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 318.742, 318.742, 318.742 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.850 - 3.250 |
| R-factor | 0.1754 |
| Rwork | 0.174 |
| R-free | 0.19320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7v5s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.645 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.370 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 43395 | 4271 |
| <I/σ(I)> | 19.9 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.9 | 13.1 |
| CC(1/2) | 0.975 | 0.878 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% (w/v) PEG 400, 0.1mM CHES, pH 9.5 |
