7V57

Structure of AdaV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 32 2 1
Unit cell lengths	139.450, 139.450, 48.103
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.850 - 2.350
R-factor	0.2105
Rwork	0.209
R-free	0.23820
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.850	2.440
High resolution limit [Å]	2.350	2.350
Rmerge	0.080	0.947
Number of reflections	22606	2359
<I/σ(I)>	28.2
Completeness [%]	99.8
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.085 M HEPES sodium pH 7.5, 1.7% v/v Polyethylene glycol 400, 1.7 M Ammonium sulfate, 15% v/v Glycerol