7V4Y

TTHA1264/TTHA1265 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	87.163, 87.163, 244.981
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.400
Rwork	0.231
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hxk 3eoq
RMSD bond length	0.010
RMSD bond angle	1.301
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (v1.6)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.980	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.141
Rpim	0.047	0.470
Number of reflections	37793	5404
<I/σ(I)>	11.9	2
Completeness [%]	99.6	99.6
Redundancy	9.8	10
CC(1/2)	0.998	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	MPD