7V4V

polylysine induce assembly of Thermotoga maritima ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-07
Detector	RAYONIX MX-225
Wavelength(s)	0.97919
Spacegroup name	P 21 21 21
Unit cell lengths	84.808, 94.495, 108.121
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.687 - 2.300
R-factor	0.2042
Rwork	0.201
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.780	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	38772
<I/σ(I)>	8.5
Completeness [%]	98.7
Redundancy	10
CC(1/2)	0.996	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	Tris-HCl pH 8.0