7V4F
Unique non-heme hydroxylase in biosynthesis of nucleoside antibiotic pathway uncover mechanism of reaction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2018-11-13 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.99 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 82.049, 82.049, 103.893 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.460 - 1.980 |
R-factor | 0.1959 |
Rwork | 0.191 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p7x |
RMSD bond length | 0.008 |
RMSD bond angle | 1.130 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.480 | 2.040 |
High resolution limit [Å] | 1.970 | 1.970 |
Number of reflections | 25823 | 2497 |
<I/σ(I)> | 33.5 | |
Completeness [%] | 100.0 | |
Redundancy | 9.8 | |
CC(1/2) | 0.905 | 0.907 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M ammonium sulfate 12% V/V PEG 8K 10 mg |